DETERMINING PROTEIN CONCENTRATION

DETERMINING PROTEIN CONCENTRATION

Results:

Set 1	Concentration BSA (mg/ml)	0.2	0.4	0.6	0.8	0.9
Set 1	Absorbance (nm)	0.164	0.205	0.221	0.231	0.231
Set 2	Concentration BSA (mg/ml)	0.2	0.4	0.6	0.8	0.9
Set 2	Absorbance (nm)	0.147	0.198	0.217	0.216	0.232

Set 1:
15μl TE buffer + 85μl H₂0 + 5 ml of diluted dyeà              absorbance = 0.038 nm
             concentration = -0.942 mg protein/ml
15μl Wash buffer + 85μl H₂0 + 5 ml of diluted dyeà
            absorbance = < 0 nm
            concentration = -1.277 mg protein/ml

Set 2:
15μl TE buffer + 85μl H₂0 + 5 ml of diluted dyeà              absorbance = 0.032 nm
             concentration = -0.995 mg protein/ml
15μl Wash buffer + 85μl H₂0 + 5 ml of diluted dyeà
            absorbance = < 0 nm
            concentration = -1.277 mg protein/ml

Theory | Purpose | Procedure