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Insertion and Isolation of Plasmids

Protein Purification

Determining Protein Concentration

SDS Gel Electrophoresis

BLAST

Conclusion

References

DETERMINING PROTEIN CONCENTRATION
 

Results: 

• Sample 1: We got a peak in the spectrum at a wavelength of 267nm with an absorbance of 0.10148.
• Sample 1 Concentration: 
  0.10148 = 30 000 M^-1cm^-1 * 0.1cm * c  ==> 
  c = 0.000034 M
  
• Sample 2: We got a peak in the spectrum at a wavelength of 264nm with an absorbance of 6.901 * 10^-2.
• Sample 2 Concentration: 
  6.901 * 10^-2 = 30 000 M^-1cm^-1 * 0.1cm * c ==>  
  c = 0.000024 M