Microtube LB/amp/ara

Micotube LB/amp

|

|

+ liquid growth media

+ liquid growth media

|

|

+ one colony of bacteria with pGlo gene from LB/amp/ara plate

+ one colony of bacteria with pGlo gene from LB/amp

|

|

--------------------------|

|--------------------------

Centrifuge
|
Pour off supernatant and examine both supernatant and pellet
|
+ 250 μl TE buffer to resuspend pellet
|
+ one drop of lysozyme
|
Freeze (0˚C) overnight
|
Spin for 10 min at top-speed in microcentrifuge
|
Pull off 250 μl of supernatant
|
Check under UV lamp
|
+ 250 μl binding buffer to 250 μl of supernatant
|
Load 250 μl of sample into drained HIC column and drain again
|
Observe column under UV lamp
|
+ 250 μl of wash buffer into column and drain
|
Observe drained solution under UV lamp
|
+ 750 μl TE buffer into column and drain
|
Observe output under UV lamp
|
Store collected samples in fridge