Insertion and Isolation of
Plasmids
PROTEIN
PURIFICATION
Results:
· Supernatant glowed under UV lamp
o Therefore, GFP protein in supernatant
· Column glowed under UV lamp
o Therefore, GFP protein is bound to column resin
· Collected wash buffer solution did not glow under UV lamp
o Therefore, GFP proteins still bound to column resin but other proteins have been release due to the high concentration of H2O in wash buffer.
· Collected TE buffer solution did glow under UV lamp
o Therefore, GFP proteins were released from column resin due to the even higher concentration of H2O in TE buffer.