The Mystery Behind
pGlo and GFP
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Insertion and Isolation of Plasmids

Protein Purification

Determining Protein Concentration

SDS Gel Electrophoresis

BLAST

Conclusion

References

 


DETERMINING STRUCTURE AND SOURCE OF A PROTEIN
 

Procedures:

   Determine the nucleotide sequence of GFP

PGlo Plasmid and Restriction Map


 

 

 

 

 

 

 

 

 

 

 

 

 Ran Blast Tutorial by inputting GFP sequence and pair-wise blast with other GFP sequence.

Theory   |   Purpose   |   Results