DETERMINING STRUCTURE AND SOURCE OF A PROTEIN 

Results:

GFP Sequence from pGlo plasmid

ATGGCTAGCAAAGGAGAAGAACTTTTCACTGGA
GTTGTCCCAATTCTTGTTGAATTAGATGGTGAT
GTTAATGGGCACAAATTTTCTGTCAGTGGAGAG
GGTGAAGGTGATGCTACATACGGAAAGCTTACC
CTTAAATTTATTTGCACTACTGGAAAACTACCT
GTTCCATGGCCAACACTTGTCACTACTTTCTCT
TATGGTGTTCAATGCTTTTCCCGTTATCCGGAT
CATATGAAACGGCATGACTTTTTCAAGAGTGCC
ATGCCCGAAGGTTATGTACAGGAACGCACTATA
TCTTTCAAAGATGACGGGAACTACAAGACGCGT
GCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAAT
CGTATCGAGTTAAAAGGTATTGATTTTAAAGAA
GATGGAAACATTCTCGGACACAAACTCGAGTAC
AACTATAACTCACACAATGTATACATCACGGCA
GACAAACAAAAGAATGGAATCAAAGCTAACTTC
AAAATTCGCCACAACATTGAAGATGGATCCGTT
CAACTAGCAGACCATTATCAACAAAATACTCCA
ATTGGCGATGGCCCTGTCCTTTTACCAGACAAC
CATTACCTGTCGACACAATCTGCCCTTTCGAAA
GATCCCAACGAAAAGCGTGACCACATGGTCCTT
CTTGAGTTTGTAACTGCTGCTGGGATTACACATGGC
ATGGATGAGCTCTACAAAT

ITALICS = Start and stop codon
BLUE = Restriction site for Nde I
GREEN = Restriction site for NHe I
PURPLE = Restriction site for Hind III
PINK = Restriction site for Xho I
RED = Restriction site for BamH I
ORANGE = Restriction site for Sal I

2EMD GFP from Aequorea Victoria

BLASTS

Using BLAST, we found pBAD-GFPuv to have the closest match to our GFP sequence.  It had the highest score of 1423 and an e-value of 0.00.

Score = 1423 bits (718)
Expect = 0.0 
Identities = 718/718 (100%) 
Strand = Plus / Plus 

We also ran a pair-wise blastn of our GFP sequence against the pBAD-GFPuv (GI=1490531).

Score = 1350 bits (702)  Expect = 0.0 Identities = 716/718 (99%) Gaps = 2/718 (0%) Strand = Plus / Plus

Theory   |   Purpose   |   Procedures