Micro tube 1

Micro tube 2

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+ 250 μl of transformation solution
(CaCl₂)

+ 250 μl of transformation solution
(CaCl₂)

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+ isolate one E. coli colony from starter plate

+ isolate one E. coli colony from starter plate

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Mix

Mix

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+ one loop (10 μl ) of pGlo

+ nothing

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Set in ice bath for 10 min
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Heat shock (42˚C) for 50 sec
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Set in ice bath for 5 – 10 min
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+ 250 μl of growth media to supply nutrients for the bacteria to feed on
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Sit at room temperature (25˚C) for 10 min

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Streak sample onto LB/Amp plate

Streak sample onto LB/Amp/Ara plate

Streak sample onto LB plate

Streak sample onto LB/Amp plate

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Incubate over night