Transformation Flowchart
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    Prepare agar plates
Prepare ampicillin
 Prepare arabinose
Rehydrate E. Coli

Micro tube 1

Micro tube 2

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Isolate one E. coli colony from LB/amp/arab plate

Isolate one E. coli colony from LB/amp plate

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Add 250 μl of transformation solution
(contains CaCl2)

Add 250 μl of transformation solution
(contains CaCl2)

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Mix well

Mix well

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Add one loop pGLO (~10 μL)

Do not add pGLO

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Put on ice ~ 10 min
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Heat shock at 42˚C for exactly 50 sec
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Put in ice water for 7 min
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Add 250 μl of growth media
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Sit at room temperature ~ 10 min

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Transfer 100 μL bacteria onto LB/amp plate, streak with loop

Transfer 100 μL bacteria onto LB/amp/arab plate, streak with loop

Transfer 100 μL bacteria onto LB plate, streak with loop

Transfer 100 μL bacteria onto LB/amp plate, streak with loop

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Incubate at 32˚C for 2 nights
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Test plates for fluorescence with blacklight
*Fluorescence indicates successful transformation*
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The rest of the procedure may be followed at the GFP Purification page.