SDS METHOD

Method for SDS PAGE

The samples were prepared first. Since the standard came ready to run it required no preparation. Hemoglobin and lysozyme were run on the gel as a way to monitor the gel and make sure we were getting accurate results. The first step in preparing these samples was to prepare a solution of the right concentration to run on the gel. A concentration of 1g/L was prepared of the hemoglobin and lysozyme. Once these samples were prepared 10μl of each sample were placed in seperate tubes. We added 3μl of dye to the lysozyme sample, the hemogobin sample and the GFP sample. Each sample was boiled for 2 minutes. The samples were then promptly injected into the chambers on the gel. . The gel was a pre-made gel obtained from Biorad. The gel was then run at 20volts for 70 min. Once the gel was done it was placed in a container along with enough b mercaptoethanol to completely cover the gel. The gel was left over night and measured the next day.

SDS PAGE apparatus

Method for SDS PAGE was taken from Monmouth College Chem330 procedure.

Results for SDS PAGE