pGLO Transformation Flow Chart

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

back to protocolFlowchart: Alternate Process: Label 2 tubes

 

 

 

 

Flowchart: Alternate Process: +pGLO Flowchart: Alternate Process: -pGLO

Flowchart: Alternate Process: add  CaCl2, chill on ice Flowchart: Alternate Process: add  CaCl2, chill on ice

Flowchart: Alternate Process: add E.Coli from starter plate Flowchart: Alternate Process: add E. Coli from starter plate

Flowchart: Alternate Process: remove both tubes from ice Flowchart: Alternate Process: add pGLO plasmid Flowchart: Alternate Process: DO NOT add pGLO plasmid

Flowchart: Alternate Process: HEAT SHOCK Flowchart: Alternate Process: place tubes back on ice Flowchart: Alternate Process: add LB to each tube and mix Flowchart: Alternate Process: Incubate at room temperature

Flowchart: Alternate Process: spread suspension on plates Flowchart: Alternate Process: +pGLO Flowchart: Alternate Process: -pGLO Flowchart: Alternate Process: spread suspension on plates

Flowchart: Connector: LB/amp + Flowchart: Connector: LB/amp/ara + Flowchart: Connector: LB/amp -

Flowchart: Connector: LB -

Flowchart: Alternate Process: Seal plates and incubate Check in 24 hours!