Ampicillin & Arabinose

"Amp" is short for ampicillin and "arab" is short for arabinose.  Ampicillin is an antibiotic, normally E. Coli cannot survive in its presence.  However, cells that have absorbed the pGLO plasmid can survive in an ampicillin environment.  This is because the particular plasmid has a gene that codes for ampicillin resistance.  Arabinose is a simple sugar molecule that "turns on" the gene that codes for GFP production.  Therefore, both +pGLO plates (LB/amp and LB/amp/arab) should exhibit growth, while only the LB/amp/arab +pGLO should be responsive to UV-light.  Furthermore, there should be no growth on the LB/amp -pGLO plate.  On the LB -pGLO plate, there should be normal growth, as this is the control for conditions in this experiment.

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