The Mystery Behind
pGlo and GFP
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Insertion and Isolation of
Plasmid

Protein Purification

Determining Protein
Concentration

SDS Gel Electrophoresis

BLAST

Conclusion

References

 


SDS Gel Electrophoresis

Results: 

Kaleidoscope Prestained Standard    
 (molecular weight given)

 

                        Myosin - 216,000
                       
$-galactosidase - 132,000

                        Bovine serum albumin - 78,000             

                        Carbonic anhydrase - 45,700

                        Soybean trypsin inhibitor - 32,500

                       Lysozyme - 18400
                       Aprotinin - 7600
 

Distances proteins traveled (mm)

Well 1

Well 2

Well 3

(standard)

Well 4

 

 

2.15

no

 

 

2.33

r

 

 

2.55

e

 

 

3.10

s

3.75

 

3.60

u

 

4.80

4.67

l

 

 

5.70

t

 

 

 

s

Protein in Well 1 - hemoglobin

            Distance traveled = 3.75 mm

            Length of protein = 31200

Protein in Well 2 - lysozyme

            Distance traveled = 4.80 mm

            Length of protein = 17901

Protein in Well 3 - standard

Protein in Well 4 - GFP

            Distance traveled = unknown

            Length of protein = unknown

§         did not have a high enough concentration of sample to run gel

 Since we know how far the standard proteins traveled we can calculate the lengths of the proteins in the other samples.

Purpose  |   Procedure   |   Theory